Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to

[Mike Bikov]

Arch Physiol Biochem. 2011 Nov 25. [Epub ahead of print]
Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance.
Gjelstad IM, Haugen F, Gulseth HL, Norheim F, Jans A, Bakke SS, Raastad T, Tjønna AE, Wisløff U, Blaak EE, Risérus U, Gaster M, Roche HM, Birkeland KI, Drevon CA.
Source

Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo , Norway.
Abstract

The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.​

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