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Ibuprofen treatment blunts early translational signalling responses in human skeletal muscle following resistance exercise
James F. Markworth , Luke Daniel Vella , Vandré Casagrande Figueiredo , David Cameron-Smith
Journal of Applied PhysiologyPublished 15 May 2014Vol. no. DOI: 10.1152/japplphysiol.01299.2013
ABSTRACT
Cyclooxygenase (COX-1 and 2) pathway derived prostaglandins (PGs) have been implicated in adaptive muscle responses to exercise, but the role of PGs in contraction-induced muscle signalling has not been determined. We investigated the effect of inhibition of COX-1 and 2 activities with the NSAID ibuprofen on human muscle signalling responses to resistance exercise. Subjects orally ingested 1200 mg ibuprofen (or placebo control) in three 400 mg doses administered ~30 min prior to, ~6 h and ~12 h following a bout of unaccustomed resistance exercise (80% 1 RM). Muscle biopsies were obtained at rest (PRE), immediately post (0 h), 3 h post, and at 24 h of recovery. In the placebo group, phosphorylation of ERK1/2 (Thr202/Tyr204), RSK (Ser380), mitogen-activated kinase 1 (Mnk1, Thr197/202), and p70S6Kinase (p70S6K, Thr421/Ser424) increased at both 0 h & 3 h post-exercise, with delayed elevation of p-p70S6K (Thr389) and p-S6 (Ser235/S36 and Ser240/244) at 3 h post. Only p-ERK (Thr202/Tyr204) remained significantly elevated in the 24 h post-exercise biopsy. Ibuprofen treatment prevented sustained elevation of MEK-ERK signalling at 3 h (p-ERK1/2, p-RSK, p-Mnk1, p70S6K Thr421/Ser424) and 24 h (p-ERK1/2) post-exercise and this was associated with supressed phosphorylation of rpS6 (Ser235/236 and Ser240/244). Early contraction induced p-Akt (Ser473) and p-p70S6K (Thr389) were not influenced by ibuprofen , but p-p70S6K (Thr389) remained elevated 24 h post-exercise only in those receiving ibuprofen treatment. Early muscle signalling responses to resistance exercise are, in part, ibuprofen sensitive, suggesting that PGs are important signalling molecules during early post-exercise recovery.
James F. Markworth , Luke Daniel Vella , Vandré Casagrande Figueiredo , David Cameron-Smith
Journal of Applied PhysiologyPublished 15 May 2014Vol. no. DOI: 10.1152/japplphysiol.01299.2013
ABSTRACT
Cyclooxygenase (COX-1 and 2) pathway derived prostaglandins (PGs) have been implicated in adaptive muscle responses to exercise, but the role of PGs in contraction-induced muscle signalling has not been determined. We investigated the effect of inhibition of COX-1 and 2 activities with the NSAID ibuprofen on human muscle signalling responses to resistance exercise. Subjects orally ingested 1200 mg ibuprofen (or placebo control) in three 400 mg doses administered ~30 min prior to, ~6 h and ~12 h following a bout of unaccustomed resistance exercise (80% 1 RM). Muscle biopsies were obtained at rest (PRE), immediately post (0 h), 3 h post, and at 24 h of recovery. In the placebo group, phosphorylation of ERK1/2 (Thr202/Tyr204), RSK (Ser380), mitogen-activated kinase 1 (Mnk1, Thr197/202), and p70S6Kinase (p70S6K, Thr421/Ser424) increased at both 0 h & 3 h post-exercise, with delayed elevation of p-p70S6K (Thr389) and p-S6 (Ser235/S36 and Ser240/244) at 3 h post. Only p-ERK (Thr202/Tyr204) remained significantly elevated in the 24 h post-exercise biopsy. Ibuprofen treatment prevented sustained elevation of MEK-ERK signalling at 3 h (p-ERK1/2, p-RSK, p-Mnk1, p70S6K Thr421/Ser424) and 24 h (p-ERK1/2) post-exercise and this was associated with supressed phosphorylation of rpS6 (Ser235/236 and Ser240/244). Early contraction induced p-Akt (Ser473) and p-p70S6K (Thr389) were not influenced by ibuprofen , but p-p70S6K (Thr389) remained elevated 24 h post-exercise only in those receiving ibuprofen treatment. Early muscle signalling responses to resistance exercise are, in part, ibuprofen sensitive, suggesting that PGs are important signalling molecules during early post-exercise recovery.
