תזונה ותוספים Effect of n-3 Fatty Acids and Antioxidants on Oxidative Stress after Exercise. Exerc

[Mike Bikov]

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Med Sci Sports Exerc. 2010 Feb 13. [Epub ahead of print]
Effect of n-3 Fatty Acids and Antioxidants on Oxidative Stress after Exercise.

McAnulty SR, Nieman DC, Fox-Rabinovich M, Duran V, McAnulty LS, Henson DA, Jin F, Landram MJ.

1Departments of Health, Leisure, and Exercise Science 2Family and Consumer Science and 3Biology 4Chemistry, Appalachian State University, Boone, NC 28608.

PURPOSE:: n-3 fatty acids are known to exert multiple beneficial effects including anti-inflammatory actions which may diminish oxidative stress. Supplementation with antioxidant vitamins has been proposed to counteract oxidative stress and improve antioxidant status. Therefore, this project investigated the effects of daily supplementation in 48 trained cyclists over 6 wks and during 3-d of continuous exercise on F2-isoprostanes (oxidative stress), plasma n-3 fatty acids, and antioxidant status (Oxygen Radical Antioxidant Capacity (ORAC) and Ferric Reducing Antioxidant Potential (FRAP)). METHODS:: Cyclists were randomized into n-3 fatty acids (N3) (N=11) (2,000 mg eicosapentaenoic acid (EPA) and 400 mg docosahexaenoic acid (DHA)), a vitamin-mineral complex (VM) (N=12) emphasizing vitamins C (2000 mg), E (800 IU), A (3000 IU) and selenium (200 ug), a vitamin-mineral and n-3 fatty acid combination (VN3) (N=13), or placebo (P) (N=12). Blood was collected at baseline and pre- and post-exercise. A 4x 3 repeated measures ANOVA was performed to test main effects. RESULTS:: After exercise, F2-isoprostanes were higher in N3 (Treatment effect P=0.014). EPA and DHA plasma values were higher after supplementation (Interaction effect P=0.001 and P=0.006, respectively) in both n-3 supplemented groups. ORAC declined similarly among all groups after exercise. FRAP exhibited significant interaction (P=0.045) and significantly increased after exercise in VN3 and VM (P<0.01). CONCLUSION:: This study indicates supplementation with n-3 fatty acids alone significantly increases F2-isoprostanes after exhaustive exercise. Lastly, antioxidant supplementation augments plasma antioxidant status and modestly attenuates but does not prevent the significant n-3 fatty acid associated increase in F2-isoprostanes post exercise.​

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